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Jumpstart Fertility jumpstart psi-blast search
A case study to demonstrate the enzyme mining protocol. A) Quercetin biosynthesis pathway (chalconeflavanone isomerase (CHI)). B) Homologue search using EC number of enzyme in UniProt database. C) Functional ImportantResidue mapping (residue block) to identify true hits (grey colour) from the alignment of homologues and the alignment <t>usedfor</t> Jumpstart PSI-BLAST search. D) FIR mapping (residue block) and phylogenetic analysis performed to identify true enzymehits (grey colour) from our proteome. E) The identified hit were further validated using tissue-based quantification by qRT-PCR(bars) and transcriptome analysis (line).
Jumpstart Psi Blast Search, supplied by Jumpstart Fertility, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jumpstart+psi-blast+search/jumpstart+psi+blast+search/pmc07528181-110-19-21
Average 90 stars, based on 1 article reviews
jumpstart psi-blast search - by Bioz Stars, 2026-08
90/100 stars

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Article Title: A knowledge-driven protocol for prediction of proteins of interest with an emphasis on biosynthetic pathways

Journal: MethodsX

doi: 10.1016/j.mex.2020.101053

A case study to demonstrate the enzyme mining protocol. A) Quercetin biosynthesis pathway (chalconeflavanone isomerase (CHI)). B) Homologue search using EC number of enzyme in UniProt database. C) Functional ImportantResidue mapping (residue block) to identify true hits (grey colour) from the alignment of homologues and the alignment usedfor Jumpstart PSI-BLAST search. D) FIR mapping (residue block) and phylogenetic analysis performed to identify true enzymehits (grey colour) from our proteome. E) The identified hit were further validated using tissue-based quantification by qRT-PCR(bars) and transcriptome analysis (line).
Figure Legend Snippet: A case study to demonstrate the enzyme mining protocol. A) Quercetin biosynthesis pathway (chalconeflavanone isomerase (CHI)). B) Homologue search using EC number of enzyme in UniProt database. C) Functional ImportantResidue mapping (residue block) to identify true hits (grey colour) from the alignment of homologues and the alignment usedfor Jumpstart PSI-BLAST search. D) FIR mapping (residue block) and phylogenetic analysis performed to identify true enzymehits (grey colour) from our proteome. E) The identified hit were further validated using tissue-based quantification by qRT-PCR(bars) and transcriptome analysis (line).

Techniques Used: Functional Assay, Residue, Blocking Assay, Quantitative RT-PCR



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Jumpstart Fertility jumpstart psi-blast search
A case study to demonstrate the enzyme mining protocol. A) Quercetin biosynthesis pathway (chalconeflavanone isomerase (CHI)). B) Homologue search using EC number of enzyme in UniProt database. C) Functional ImportantResidue mapping (residue block) to identify true hits (grey colour) from the alignment of homologues and the alignment <t>usedfor</t> Jumpstart PSI-BLAST search. D) FIR mapping (residue block) and phylogenetic analysis performed to identify true enzymehits (grey colour) from our proteome. E) The identified hit were further validated using tissue-based quantification by qRT-PCR(bars) and transcriptome analysis (line).
Jumpstart Psi Blast Search, supplied by Jumpstart Fertility, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jumpstart+psi-blast+search/jumpstart+psi+blast+search/pmc07528181-110-19-21
Average 90 stars, based on 1 article reviews
jumpstart psi-blast search - by Bioz Stars, 2026-08
90/100 stars
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A case study to demonstrate the enzyme mining protocol. A) Quercetin biosynthesis pathway (chalconeflavanone isomerase (CHI)). B) Homologue search using EC number of enzyme in UniProt database. C) Functional ImportantResidue mapping (residue block) to identify true hits (grey colour) from the alignment of homologues and the alignment usedfor Jumpstart PSI-BLAST search. D) FIR mapping (residue block) and phylogenetic analysis performed to identify true enzymehits (grey colour) from our proteome. E) The identified hit were further validated using tissue-based quantification by qRT-PCR(bars) and transcriptome analysis (line).

Journal: MethodsX

Article Title: A knowledge-driven protocol for prediction of proteins of interest with an emphasis on biosynthetic pathways

doi: 10.1016/j.mex.2020.101053

Figure Lengend Snippet: A case study to demonstrate the enzyme mining protocol. A) Quercetin biosynthesis pathway (chalconeflavanone isomerase (CHI)). B) Homologue search using EC number of enzyme in UniProt database. C) Functional ImportantResidue mapping (residue block) to identify true hits (grey colour) from the alignment of homologues and the alignment usedfor Jumpstart PSI-BLAST search. D) FIR mapping (residue block) and phylogenetic analysis performed to identify true enzymehits (grey colour) from our proteome. E) The identified hit were further validated using tissue-based quantification by qRT-PCR(bars) and transcriptome analysis (line).

Article Snippet: C) Functional ImportantResidue mapping (residue block) to identify true hits (grey colour) from the alignment of homologues and the alignment usedfor Jumpstart PSI-BLAST search.

Techniques: Functional Assay, Residue, Blocking Assay, Quantitative RT-PCR